Spinning-Disk Microscopy Systems

Resolution doubling in fluorescence microscopy with confocal spinning-disk image scanning microscopy.

We demonstrate how a conventional confocal spinning-disk (CSD) microscope can be converted into a doubly resolving image scanning microscopy (ISM) system without changing any part of its optical or mechanical elements. Making use of the intrinsic properties of a CSD microscope, we illuminate stroboscopically, generating an array of excitation foci that are moved across the sample by varying the...

The BioCD Spinning-Disk Interferometry

High speed interferometry on a spinning disk opens new opportunities for sensitive material metrology. One promising application is fast molecular screening in diagnostic medicine.

Visualizing stromal cell dynamics in different tumor microenvironments by spinning disk confocal microscopy.

The tumor microenvironment consists of stromal cells and extracellular factors that evolve in parallel with carcinoma cells. To gain insights into the activities of stromal cell populations, we developed and applied multicolor imaging techniques to analyze the behavior of these cells within different tumor microenvironments in the same live mouse. We found that regulatory T-lymphocytes (Tregs) ...

Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging.

A recent key requirement in life sciences is the observation of biological processes in their natural in vivo context. However, imaging techniques that allow fast imaging with higher resolution in 3D thick specimens are still limited. Spinning disk confocal microscopy using a Yokogawa Confocal Scanner Unit, which offers high-speed multipoint confocal live imaging, has been found to have wide ut...

Simple method to improve image contrast in spinning disk microscopy using adaptive optics